First rd out of magazine is chambered with a different BCG speed / momentum. Many times this causes round to be out of core group.
Last round out of magazine, no ammo pushing up on bottom of bolt carrier so there is slightly different support on case head. Many times this causes round to be out of core group.
Both are well documented.
I test AR loads as they will be used; how load behaves as a single shot isn’t the same as how ammo behaves when used as a gas operated system. Experienced this a time or two, seen this happen to others many more times.
Test the whole system (rifle, ammo, magazines, optic, bipod & rear bag or slung up) in the way you’ll be using system. IMHO, you’ll be farther ahead, have the system better optimized testing how system will be used majority of the time than if test ammo one way but actual use is significantly different.
140 SMK, 6.5 Creed gas gun you’ll be a little slow with 4831sc for velocity, port pressure will be a little higher even though peak pressure is lower.
How long is gas system?
How heavy is buffer?
10 twist barrel? If really a 10 twist, I’d be looking at lighter bullets as 140s, even the 140 SMK w/8-10 ogive radius, probably won’t be stabilized with a 10 twist bbl.
140s do well with H4340, possibly H414/Win 760 (too temp sensitive for my liking), possibly IMR4451, maybe IMR4955. AA4350 is usually a smidgen faster in burn rate than H4350, might work well in gas gun application w/140 SMK.
I haven’t tried StaBall 6.5 yet, not a fan of ball powders if I can avoid them.
130 AR Hybrid is the Goldie Locks bullet for BC / velocity for 6.5 Creed gas gun, in my testing / experience. 130 TMK / ELD-M probably in mix also, I’ve not had a reason to stray from the Berger.